A healthy Microbiota is an indispensable ally in all the various stages of life.
Eye OphthalResearch, in collaboration with Allergoline, offers you the opportunity to evaluate the Microbiota of your patients.
Single shipping method, valid for both swabs, genetic analyzes, and blood samples for food intolerance investigations.
Shipping to be paid by Allergoline:
book collection in ASSIGNED PORT using the subscription code 107676 in the name of Allergoline Biotech & Research , with delivery in Via delle Caravelle, 11, 56021, Cascina (PI) , using the web page www.sda.it (by clicking on the image) - > BOOK A RETREAT and filling in the fields as shown in the image
In the case of sending multiple withdrawals, please combine everything in a single shipment. Insert in the envelope both the exam request (complete in all its signed parts) and the sample to be analyzed on which the patient's name must be written.
Shipping with ORDINARY MAIL
It is also possible to send the withdrawal by ordinary mail, in this case the shipment is charged to the sender who can choose the most appropriate form (priority mail - registered mail - registered mail 1, etc ...).
The shipping address is Allergoline Biotech & Research - Via delle Caravelle, 11, 56021, Cascina (PI)
FECAL SAMPLING INSTRUCTIONS:
The kit is used to take stool samples to be sent to the laboratory for genetic analysis of the fecal microbiota. Once sealed, the swab protects the integrity of the microbial DNA for months when kept at room temperature. Withdrawal is simple, fast, safe and can also be done easily from home. The stools must be early in the morning, at least seven days after the last antibiotic therapy, do not take laxatives, antidiarrheals, antacids. The test consists of a metagenomic analysis of the intestinal microflora and is performed using Next Generation Sequencing (NGS) technology. The DNA analysis of the fecal microbiota allows to identify a series of bacterial and fungal phylums capable of influencing the composition and balance of the intestinal microbiota, correlating them with the main physiological functions of the host and identifying and characterizing any situations of intestinal dysbiosis .
The kit contains:
The test is very useful for preventive purposes, but also in subjects in which certain problems have already arisen, as well as in patients in particular stages of life such as infancy and aging, pregnancy, breastfeeding, to direct a precise food plan and / or an approach therapeutic
read the instructions carefully before using the kit.
In order to obtain reliable results, only use samples taken from fresh stool.
Empty your bladder before taking the sample.
Collect a stool sample free of urine or water from the toilet.
You may need to use some toilet paper or tissue.
Samples not collected with the methods and quantities indicated may not be analysable
Intended for the collection of microbial cells.
The swab is for single use and for external use only.
Store at room temperature.
Use only if the package is intact and sealed.
In case of need, or if the kit is missing some component, please contact customer service:
Do not ingest the stabilizer solution.
If the stabilizing solution comes into contact with the skin or eyes, repeatedly rinse with plenty of water.
The kit should only be used by adults.
Keep out of reach of children - the casing could be a potential choking hazard.
Instructions for fecal sampling
Instructions for buccal sampling
The sampling is performed with CYTOBRUSH to recover more material from which to extract DNA for the execution of genetic tests.
The withdrawal method is as follows:
Open the pouch and remove the sterile brush.
Pass the brush inside the right and left cheeks for about 30 seconds per side.
Place the brush in the same case and write the patient's surname and first name on the back.
If possible close with adhesive tape, otherwise it is sufficient to fold the flap of the case.
The sample can be kept for a long time at room temperature. It is therefore not necessary to put in place any particular method of conservation even if the sample is sent after several days.
For any doubt and / or further clarification send an email here:
Examples of reports and results
Insights on Polymorphisms
MICROBIOTA-INFLUENCE OF DNA
PTPN2- PROTEIN TIROSIN PHOSPHATASE N2
Chronic intestinal inflammation
The gut microbiota is critical to our health, and well-balanced interactions between the host's immune system and the microbiota are essential to prevent chronic inflammatory bowel disease (IBD).
Genomic association studies have identified several genes related to greater susceptibility to these diseases. Among these, a variant of the non-receptorial protein tyrosine phosphatase (PTP) of tpo 2 (PTPN2) is associated with a reduced risk of developing IBD.
PTPs are proteins involved in the response of B and T lymphocyte receptors and play an important role in immune cell function and intestinal homeostasis.
The loss of PTPN2 functionality is associated with a greater induction of T helper lymphocytes, which play a fundamental role in the immune system, producing a specific response for each type of substance considered foreign. The alteration in the production of T helper lymphocytes leads to an increased susceptibility to intestinal inflammation predisposing to the development of IBD.
The dysfunction of the non-receptor tyrosine phosphatase protein type 2 (PTPN2) also contributes to alterations in the composition of the intestinal microbiota, a condition which in turn predisposes to the pathogenesis of inflammatory bowel disease (IBD).
Recent scientific studies (Sharp et al., 2015 and Yilmaz et al, 2018), in fact, have shown how subjects with the presence of the genetic variant PTPN2 have an altered intestinal bacterial composition, especially as regards the Clostridialeceae and Lachnospiraceae families, which correlates with IBD.
Specifically, the studies report an increase in Roseburia spp. (Lachnospiraceae) and Ruminococcus spp. (Lachnospiraceae) in patients with ulcerative colitis (UC), compared to controls (healthy individuals). In patients with Crohn's disease (CD), however, a reduction of Faecalibacterium spp was identified. (Clostridiaceae), Bilophila spp. (Desulfovibrionaceae), Coprococcus spp. (Lachnospiraceae) and species belonging to the Erysipelotrichaeceae, Clostridiaceae and Ruminococcaceae families, while bacterial species belonging to the Bacteroidaceae family were increased compared to controls (healthy individuals).
In conclusion, the genetic variant PTPN2 was found in association with alterations of the intestinal microflora and an increased risk of developing conditions such as Crohn's disease, diabetes, obesity, colitis and rheumatoid arthritis.